Hotspot

psaC

SSC · 119529-119774 · 246 bp

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Pi 0.466

MarkerSeek score 76.20

Primer available yes

Label psaC

Feature Metadata

label_name: psaC
feature_type: gene
region: SSC
length_bp: 246
variable_sites: 104
pi: 0.466
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 76.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC_p1	AAAACTCGGGGAACTCATCG	TCTTTCCTCCCCACATGGAT	50.0	50.0	57.9	58.0	949 bp	0.5	59.1
psaC_p2	CTCGGGGAACTCATCGATTC	TCTTTCCTCCCCACATGGAT	55.0	50.0	57.9	58.0	945 bp	0.5	58.8
psaC_p3	AAAACTCGGGGAACTCATCG	GAGACGTTTCTTTCCTCCCC	50.0	55.0	57.9	57.9	957 bp	0.5	58.5
psaC_p4	AAAACTCGGGGAACTCATCG	TTCCAAAGCCACAGGATACG	50.0	50.0	57.9	57.9	1104 bp	0.5	58.4
psaC_p5	CTCGGGGAACTCATCGATTC	GAGACGTTTCTTTCCTCCCC	55.0	55.0	57.9	57.9	953 bp	0.5	58.1