Hotspot

ndhH

SSC · 113939-115120 · 1182 bp

Back to Silphium

Pi 0.494

MarkerSeek score 80.30

Primer available yes

Label ndhH

Feature Metadata

label_name: ndhH
feature_type: gene
region: SSC
length_bp: 1182
variable_sites: 487
pi: 0.494
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.83
markerseek_score: 80.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhH_p1	GCAAGTAACCCAATGCACAC	CGTTGTTGTATACCCGCAGA	50.0	50.0	57.9	58.0	2032 bp	0.5	59.5
ndhH_p2	TTTCTGCTGTCCGTGATCTG	CGTTGTTGTATACCCGCAGA	50.0	50.0	57.9	58.0	2070 bp	0.5	59.3
ndhH_p3	TCAACGGTTGCTGGCTTATT	CGTTGTTGTATACCCGCAGA	45.0	50.0	58.1	58.0	1549 bp	0.5	59.2
ndhH_p4	GCAAGTAACCCAATGCACAC	CCTGACATAAGAAGGCCGAC	50.0	55.0	57.9	58.1	2296 bp	0.5	59.0
ndhH_p5	GCAAGTAACCCAATGCACAC	GACATAAGAAGGCCGACAGG	50.0	55.0	57.9	58.1	2293 bp	0.5	59.0