Hotspot

ndhA

SSC · 115122-117289 · 2168 bp

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Pi 0.452

MarkerSeek score 79.80

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2168
variable_sites: 845
pi: 0.452
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.86
markerseek_score: 79.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	CGGCCACCGGGATTTATTAA	TGACCAATATACCTTGCGGC	50.0	50.0	58.0	58.0	2438 bp	0.5	59.5
ndhA_p2	TCAACAGGCCCTAGAAGGAA	TGACCAATATACCTTGCGGC	50.0	50.0	58.0	58.0	2676 bp	0.5	59.4
ndhA_p3	TTCAACAGGCCCTAGAAGGA	TGACCAATATACCTTGCGGC	50.0	50.0	58.0	58.0	2677 bp	0.5	59.4
ndhA_p4	GGCAAAAAGAGGGGGATTCA	TGACCAATATACCTTGCGGC	50.0	50.0	58.1	58.0	2749 bp	0.5	59.2
ndhA_p5	TGGCAAAAAGAGGGGGATTC	TGACCAATATACCTTGCGGC	50.0	50.0	58.1	58.0	2750 bp	0.5	59.2