Hotspot

ccsA

SSC · 121656-122624 · 969 bp

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Pi 0.492

MarkerSeek score 81.70

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 969
variable_sites: 422
pi: 0.492
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 81.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	GTTTTTGATTCTGGACCGCG	GTGAAATCGGTAGACACGCT	50.0	50.0	58.0	58.0	1550 bp	0.5	59.6
ccsA_p2	GTTTTTGATTCTGGACCGCG	TGAAATCGGTAGACACGCTG	50.0	50.0	58.0	58.0	1549 bp	0.5	59.6
ccsA_p3	GTTTTTGATTCTGGACCGCG	AATTTTGGTGGTGGGGAGTC	50.0	50.0	58.0	58.0	2090 bp	0.5	59.6
ccsA_p4	CATGGCTTTATTGGTGCTGC	GTGAAATCGGTAGACACGCT	50.0	50.0	58.1	58.0	1898 bp	0.5	59.4
ccsA_p5	CATGGCTTTATTGGTGCTGC	TGAAATCGGTAGACACGCTG	50.0	50.0	58.1	58.0	1897 bp	0.5	59.4