Hotspot

rpl32-ndhF

SSC · 123748-124734 · 987 bp

Back to Silphium

Pi 0.444

MarkerSeek score 80.20

Primer available yes

Label rpl32-ndhF

Feature Metadata

label_name: rpl32-ndhF
feature_type: igs
region: SSC
length_bp: 987
variable_sites: 363
pi: 0.444
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.83
markerseek_score: 80.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-ndhF_p1	GACTCCCCACCACCAAAATT	GTTGCCGTCGGAAAAAGAAG	50.0	50.0	58.0	58.0	1509 bp	0.5	59.7
rpl32-ndhF_p2	GACTCCCCACCACCAAAATT	TGCCGTCGGAAAAAGAAGAA	50.0	45.0	58.0	58.0	1507 bp	0.5	59.6
rpl32-ndhF_p3	GACTCCCCACCACCAAAATT	TTGCCGTCGGAAAAAGAAGA	50.0	45.0	58.0	58.0	1508 bp	0.5	59.6
rpl32-ndhF_p4	AATAAGACTCCCCACCACCA	GTTGCCGTCGGAAAAAGAAG	50.0	50.0	57.9	58.0	1514 bp	0.5	59.3
rpl32-ndhF_p5	ATAAGACTCCCCACCACCAA	GTTGCCGTCGGAAAAAGAAG	50.0	50.0	57.9	58.0	1513 bp	0.5	59.3