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trnL-UAG-rpl32

SSC · 122814-123582 · 769 bp

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Pi 0.448

MarkerSeek score 82.70

Primer available yes

Label trnL-UAG-rpl32

Feature Metadata

label_name: trnL-UAG-rpl32
feature_type: igs
region: SSC
length_bp: 769
variable_sites: 309
pi: 0.448
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 82.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAG-rpl32_p1	AGCGTGTCTACCGATTTCAC	GGATATGAGGCGGCGTTAAA	50.0	50.0	58.0	58.1	895 bp	0.5	58.9
trnL-UAG-rpl32_p2	CAGCGTGTCTACCGATTTCA	GGATATGAGGCGGCGTTAAA	50.0	50.0	58.0	58.1	896 bp	0.5	58.9
trnL-UAG-rpl32_p3	GAAGTAGCAAAGCCTTGGGT	GGATATGAGGCGGCGTTAAA	50.0	50.0	58.1	58.1	1398 bp	0.5	58.3
trnL-UAG-rpl32_p4	TGATCCACAAAGAAGAGCCG	GGATATGAGGCGGCGTTAAA	50.0	50.0	57.9	58.1	1516 bp	0.5	58.2
trnL-UAG-rpl32_p5	TCCTAAGAGCAGCGTGTCTA	GGATATGAGGCGGCGTTAAA	50.0	50.0	57.9	58.1	905 bp	0.5	58.0