Hotspot

ndhD-ccsA

SSC · 121398-121655 · 258 bp

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Pi 0.539

MarkerSeek score 71.40

Primer available yes

Label ndhD-ccsA

Feature Metadata

label_name: ndhD-ccsA
feature_type: igs
region: SSC
length_bp: 258
variable_sites: 103
pi: 0.539
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.74
markerseek_score: 71.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-ccsA_p1	CTCCCGGCCAACGTAATTTA	AGCAAATTCCGCAATTGTGG	50.0	45.0	58.0	57.9	999 bp	0.5	59.1
ndhD-ccsA_p2	CTCCCGGCCAACGTAATTTA	ACCCAAGGCTTTGCTACTTC	50.0	50.0	58.0	58.1	1523 bp	0.5	59.1
ndhD-ccsA_p3	GTTTTTGATTCTGGACCGCG	AGCAAATTCCGCAATTGTGG	50.0	45.0	58.0	57.9	523 bp	0.5	59.0
ndhD-ccsA_p4	GTTTTTGATTCTGGACCGCG	ACCCAAGGCTTTGCTACTTC	50.0	50.0	58.0	58.1	1047 bp	0.5	59.0
ndhD-ccsA_p5	CTCCCGGCCAACGTAATTTA	CGGCTCTTCTTTGTGGATCA	50.0	50.0	58.0	57.9	1405 bp	0.5	59.0