Hotspot

ycf1-rps15

SSC · 113175-113569 · 395 bp

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Pi 0.476

MarkerSeek score 79.40

Primer available yes

Label ycf1-rps15

Feature Metadata

label_name: ycf1-rps15
feature_type: igs
region: SSC
length_bp: 395
variable_sites: 178
pi: 0.476
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 79.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-rps15_p1	AATTATGGAACCTGCCCGTC	CCGCAGACCTCTCTGAGATA	50.0	55.0	57.9	58.0	1074 bp	0.5	59.4
ycf1-rps15_p2	AATTATGGAACCTGCCCGTC	AACACCATGCATTGATGGGT	50.0	45.0	57.9	58.1	1350 bp	0.5	59.1
ycf1-rps15_p3	TATGGAACCTGCCCGTCTAT	CCGCAGACCTCTCTGAGATA	50.0	55.0	57.9	58.0	1071 bp	0.5	59.0
ycf1-rps15_p4	AATTATGGAACCTGCCCGTC	AATAAGCCAGCAACCGTTGA	50.0	45.0	57.9	58.1	1111 bp	0.5	58.8
ycf1-rps15_p5	TATGGAACCTGCCCGTCTAT	AACACCATGCATTGATGGGT	50.0	45.0	57.9	58.1	1347 bp	0.5	58.8