Hotspot

accD

LSC · 56736-58673 · 1938 bp

Back to Silene

Pi 0.12
MarkerSeek score 72.30
Primer available yes
Label accD

Feature Metadata

label_name
accD
feature_type
gene
region
LSC
length_bp
1938
variable_sites
469
pi
0.12
unique_haplotype_count
27
primer_available
yes
alignment_reliability
0.74
markerseek_score
72.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
accD_p1 AATTTGACATAGCGCGGGAA CCATTGCAATTGCCGGAAAT 45.0 45.0 58.3 58.0 80-2534 bp 0.931034 87.0
accD_p2 ATTTGACATAGCGCGGGAAA CCATTGCAATTGCCGGAAAT 45.0 45.0 58.3 58.0 80-2533 bp 0.931034 87.0
accD_p3 TCCCCAATGAATCCTACGGA CCATTGCAATTGCCGGAAAT 50.0 45.0 57.8 58.0 80-2787 bp 0.896552 86.2
accD_p4 TCCCCAATGAATCCTACGGA ACAGAGATTTTGGCCACAGG 50.0 50.0 57.8 58.1 80-2850 bp 0.896552 85.7
accD_p5 AAATTTGACATAGCGCGGGA CCATTGCAATTGCCGGAAAT 45.0 45.0 58.3 58.0 80-2535 bp 0.896552 85.6