Hotspot

trnN-GUU-ycf1

IRb · 107591-107946 · 356 bp

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Pi 0.112
MarkerSeek score 60.10
Primer available yes
Label trnN-GUU-ycf1

Feature Metadata

label_name
trnN-GUU-ycf1
feature_type
igs
region
IRb
length_bp
356
variable_sites
173
pi
0.112
unique_haplotype_count
13
primer_available
yes
alignment_reliability
0.79
markerseek_score
60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnN-GUU-ycf1_p1 GATCAAAGCGAGCCTCTTCA AGGAAGAGATAAGAGGGCCC 50.0 55.0 58.0 57.9 80-915 bp 0.931034 88.2
trnN-GUU-ycf1_p2 GATCAAAGCGAGCCTCTTCA GGAAGAGATAAGAGGGCCCT 50.0 55.0 58.0 57.9 80-914 bp 0.931034 88.2
trnN-GUU-ycf1_p3 GCGAGCCTCTTCATTCTGAA AGGAAGAGATAAGAGGGCCC 50.0 55.0 58.0 57.9 80-908 bp 0.344828 64.8
trnN-GUU-ycf1_p4 GCGAGCCTCTTCATTCTGAA GGAAGAGATAAGAGGGCCCT 50.0 55.0 58.0 57.9 80-907 bp 0.344828 64.8
trnN-GUU-ycf1_p5 AAGCGAGCCTCTTCATTCTG GGAAGAGATAAGAGGGCCCT 50.0 55.0 58.0 57.9 80-909 bp 0.344828 64.7