Hotspot

ndhC-trnV-UAC

LSC · 49680-50794 · 1115 bp

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Pi 0.0447
MarkerSeek score 81.30
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1115
variable_sites
239
pi
0.0447
unique_haplotype_count
28
primer_available
yes
alignment_reliability
0.92
markerseek_score
81.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TGTCCAGAAAATGTCATATTCA GGTTCGAATCCGTATAGCCC 31.8 55.0 53.4 57.9 80-1173 bp 0.931034 49.2
ndhC-trnV-UAC_p2 TGTCCAGAAAATGTCATATTCA CGGTTCGAATCCGTATAGCC 31.8 55.0 53.4 58.3 80-1174 bp 0.931034 49.2
ndhC-trnV-UAC_p3 TGTCCAGAAAATGTCATATTCA ACTGCATGTTGGGTCTTTGA 31.8 45.0 53.4 57.6 80-1254 bp 0.931034 49.2
ndhC-trnV-UAC_p4 AAAAATGTCCAGAAAATGTCA GGTTCGAATCCGTATAGCCC 28.6 55.0 52.1 57.9 80-1178 bp 0.931034 49.2
ndhC-trnV-UAC_p5 TGTCCAGAAAATGTCATATTCAT GGTTCGAATCCGTATAGCCC 30.4 55.0 54.1 57.9 80-1173 bp 0.931034 49.2