Hotspot

psaA-ycf3

LSC · 40306-41299 · 994 bp

Back to Silene

Pi 0.0541
MarkerSeek score 80.90
Primer available yes
Label psaA-ycf3

Feature Metadata

label_name
psaA-ycf3
feature_type
igs
region
LSC
length_bp
994
variable_sites
238
pi
0.0541
unique_haplotype_count
27
primer_available
yes
alignment_reliability
0.94
markerseek_score
80.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaA-ycf3_p1 GGCACTAGGTCCAATGTGAG ATTGCAGAGGCTTGGTTTGA 55.0 45.0 58.0 58.0 80-1639 bp 1.0 91.8
psaA-ycf3_p2 GGCACTAGGTCCAATGTGAG TGAAATTGCAGAGGCTTGGT 55.0 45.0 58.0 58.0 80-1643 bp 1.0 91.8
psaA-ycf3_p3 GGCACTAGGTCCAATGTGAG TTGCAGAGGCTTGGTTTGAT 55.0 45.0 58.0 58.0 80-1638 bp 1.0 91.8
psaA-ycf3_p4 GCATCAGCATGTAGGTTCCA ATTGCAGAGGCTTGGTTTGA 50.0 45.0 58.0 58.0 80-1461 bp 1.0 91.7
psaA-ycf3_p5 GCATCAGCATGTAGGTTCCA TTGCAGAGGCTTGGTTTGAT 50.0 45.0 58.0 58.0 80-1460 bp 1.0 91.7