Hotspot

trnE-UUC-trnT-GGU

LSC · 29595-30150 · 556 bp

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Pi 0.0443
MarkerSeek score 79.20
Primer available yes
Label trnE-UUC-trnT-GGU

Feature Metadata

label_name
trnE-UUC-trnT-GGU
feature_type
igs
region
LSC
length_bp
556
variable_sites
137
pi
0.0443
unique_haplotype_count
28
primer_available
yes
alignment_reliability
0.98
markerseek_score
79.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnE-UUC-trnT-GGU_p1 AGATGTCCTGAACCGCTAGA ACTCTATTCCGCGTGCAAAT 50.0 45.0 57.9 58.0 80-778 bp 0.965517 89.3
trnE-UUC-trnT-GGU_p2 AGATGTCCTGAACCGCTAGA CCATGGCGTTACTCTACCAC 50.0 55.0 57.9 58.1 80-687 bp 0.965517 88.9
trnE-UUC-trnT-GGU_p3 AGAGATGTCCTGAACCGCTA GAACCGATGACTTACGCCTT 50.0 50.0 57.9 58.0 80-710 bp 0.931034 88.0
trnE-UUC-trnT-GGU_p4 AGATGTCCTGAACCGCTAGA GAACCGATGACTTACGCCTT 50.0 50.0 57.9 58.0 80-708 bp 0.931034 88.0
trnE-UUC-trnT-GGU_p5 AGAGATGTCCTGAACCGCTA ACTCTATTCCGCGTGCAAAT 50.0 45.0 57.9 58.0 80-780 bp 0.931034 87.9