Hotspot

petN-psbM

LSC · 27811-28359 · 549 bp

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Pi 0.0425
MarkerSeek score 80.20
Primer available yes
Label petN-psbM

Feature Metadata

label_name
petN-psbM
feature_type
igs
region
LSC
length_bp
549
variable_sites
99
pi
0.0425
unique_haplotype_count
27
primer_available
yes
alignment_reliability
0.96
markerseek_score
80.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petN-psbM_p1 ATTGTAAGTCTCGCTTGGGC AAAACGGTCAGCCAAAATGA 50.0 40.0 58.3 56.4 80-694 bp 0.965517 76.1
petN-psbM_p2 ATGGGGAAGAAGTGGACTCT AAAACGGTCAGCCAAAATGA 50.0 40.0 57.6 56.4 80-632 bp 0.965517 75.4
petN-psbM_p3 ATTGTAAGTCTCGCTTGGGC AAACGGTCAGCCAAAATGAT 50.0 40.0 58.3 56.2 80-693 bp 0.965517 74.2
petN-psbM_p4 ATTGTAAGTCTCGCTTGGGC AACGGTCAGCCAAAATGATT 50.0 40.0 58.3 56.2 80-692 bp 0.965517 74.2
petN-psbM_p5 ATTGTAAGTCTCGCTTGGGC AAAAACGGTCAGCCAAAATGA 50.0 38.1 58.3 57.2 80-695 bp 0.965517 74.1