Hotspot

psbK-psbI

LSC · 7178-7616 · 439 bp

Back to Silene

Pi 0.0473
MarkerSeek score 81.70
Primer available yes
Label psbK-psbI

Feature Metadata

label_name
psbK-psbI
feature_type
igs
region
LSC
length_bp
439
variable_sites
102
pi
0.0473
unique_haplotype_count
25
primer_available
yes
alignment_reliability
0.97
markerseek_score
81.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbK-psbI_p1 GTTTGGCAAGCTGCTGTAAG ACGCCCCGGATCATTAGATA 50.0 50.0 57.9 58.1 80-618 bp 1.0 91.0
psbK-psbI_p2 GTTTGGCAAGCTGCTGTAAG TACGCCCCGGATCATTAGAT 50.0 50.0 57.9 58.1 80-619 bp 1.0 91.0
psbK-psbI_p3 GTTTGGCAAGCTGCTGTAAG TTACGCCCCGGATCATTAGA 50.0 50.0 57.9 58.3 80-620 bp 1.0 89.2
psbK-psbI_p4 TGGCAAGCTGCTGTAAGTTT ACGCCCCGGATCATTAGATA 45.0 50.0 58.3 58.1 80-615 bp 1.0 89.0
psbK-psbI_p5 TGGCAAGCTGCTGTAAGTTT TACGCCCCGGATCATTAGAT 45.0 50.0 58.3 58.1 80-616 bp 1.0 89.0