Hotspot

rps16-trnQ-UUG

LSC · 5924-6619 · 696 bp

Back to Silene

Pi 0.0519
MarkerSeek score 85.80
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
696
variable_sites
171
pi
0.0519
unique_haplotype_count
28
primer_available
yes
alignment_reliability
0.96
markerseek_score
85.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 80-810 bp 1.0 90.3
rps16-trnQ-UUG_p2 TCATGTCCTTCAAGTCGCAC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.2 58.1 80-821 bp 1.0 89.8
rps16-trnQ-UUG_p3 AAGTCGCACGTTGCTTTCTA TAAGGCATCGGGTTTTGGTC 45.0 50.0 58.1 58.2 80-840 bp 1.0 89.5
rps16-trnQ-UUG_p4 TCATGTCCTTCAAGTCGCAC TAAGGCATCGGGTTTTGGTC 50.0 50.0 58.2 58.2 80-851 bp 1.0 89.0
rps16-trnQ-UUG_p5 AAGTCGCACGTTGCTTTCTA ATAGAAATCGAAGTGGGGCG 45.0 50.0 58.1 57.8 80-871 bp 1.0 89.0