Hotspot

clpP

LSC · 74736-76881 · 2146 bp

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Pi 0.00656
MarkerSeek score 49.20
Primer available yes
Label clpP

Feature Metadata

label_name
clpP
feature_type
gene
region
LSC
length_bp
2146
variable_sites
27
pi
0.00656
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.99
markerseek_score
49.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
clpP_p1 CTAGTACATGTTCCTCGGCG ATACCAAGGCAAACCCATGG 55.0 50.0 57.9 58.1 2880-2887 bp 1.0 79.4
clpP_p2 TTTTCTCAGCCCATGATCGG ATACCAAGGCAAACCCATGG 50.0 50.0 58.0 58.1 2926 bp 0.25 48.6
clpP_p3 TTTTCTCAGCCCATGATCGG GCGACCGGGATCATTCAATA 50.0 50.0 58.0 57.8 2962 bp 0.25 48.3
clpP_p4 TTTTCTCAGCCCATGATCGG CCTTCCCTTTTCGGTGGATT 50.0 50.0 58.0 57.8 2850 bp 0.25 47.9
clpP_p5 TTTTCTCAGCCCATGATCGG CGATACCAAGGCAAACCCAT 50.0 50.0 58.0 58.2 2928 bp 0.25 47.8