Hotspot

cemA-petA

LSC · 66189-66425 · 237 bp

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Pi 0.0115
MarkerSeek score 65.50
Primer available yes
Label cemA-petA

Feature Metadata

label_name
cemA-petA
feature_type
igs
region
LSC
length_bp
237
variable_sites
5
pi
0.0115
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.98
markerseek_score
65.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
cemA-petA_p1 CGTGTATCTCCGTCACTTGT GAAATGGATGCCCCGGTTAT 50.0 50.0 57.4 58.0 369-383 bp 1.0 78.9
cemA-petA_p2 CGTGTATCTCCGTCACTTGT GCGATACGGAAATGGATCGA 50.0 50.0 57.4 57.9 335-349 bp 1.0 78.9
cemA-petA_p3 CGTGTATCTCCGTCACTTGT AGCGATACGGAAATGGATCG 50.0 50.0 57.4 57.9 336-350 bp 1.0 78.8
cemA-petA_p4 CGTGTATCTCCGTCACTTGT GCATTTGAAATGGATGCCCC 50.0 50.0 57.4 58.0 375-389 bp 1.0 78.6
cemA-petA_p5 CGTGTATCTCCGTCACTTGT CATTTGAAATGGATGCCCCG 50.0 50.0 57.4 57.8 374-388 bp 1.0 77.1