Hotspot

trnT-GGU-psbD

LSC · 33001-34489 · 1489 bp

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Pi 0.0145
MarkerSeek score 62.80
Primer available yes
Label trnT-GGU-psbD

Feature Metadata

label_name
trnT-GGU-psbD
feature_type
igs
region
LSC
length_bp
1489
variable_sites
42
pi
0.0145
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.99
markerseek_score
62.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-psbD_p1 GTGGTAGAGTAACGCCATGG AAAAACGAACCGGTCCCTAC 55.0 50.0 58.1 58.1 1637-1681 bp 1.0 81.4
trnT-GGU-psbD_p2 GTGGTAGAGTAACGCCATGG CAAAAACGAACCGGTCCCTA 55.0 50.0 58.1 58.1 1638-1682 bp 1.0 81.4
trnT-GGU-psbD_p3 TCAGTGGTAGAGTAACGCCA AAAAACGAACCGGTCCCTAC 50.0 50.0 58.1 58.1 1640-1684 bp 1.0 81.3
trnT-GGU-psbD_p4 TCAGTGGTAGAGTAACGCCA CAAAAACGAACCGGTCCCTA 50.0 50.0 58.1 58.1 1641-1685 bp 1.0 81.3
trnT-GGU-psbD_p5 ACGGCCCTTTTAACTCAGTG AAAAACGAACCGGTCCCTAC 50.0 50.0 58.1 58.1 1654-1698 bp 1.0 81.1