Hotspot

trnK-UUU-rps16

LSC · 4467-5336 · 870 bp

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Pi 0.0106
MarkerSeek score 56.50
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
870
variable_sites
18
pi
0.0106
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.97
markerseek_score
56.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 CCTTTCAGGATCAGTCGTGG CCCAATGAGCCGTTTATCGA 55.0 50.0 58.0 58.1 1232-1273 bp 1.0 83.0
trnK-UUU-rps16_p2 CCTTTCAGGATCAGTCGTGG TCCCAATGAGCCGTTTATCG 55.0 50.0 58.0 58.1 1233-1274 bp 1.0 83.0
trnK-UUU-rps16_p3 CCTTTCAGGATCAGTCGTGG GCCGTACGAGGAGAAAACTT 55.0 50.0 58.0 57.9 1293-1334 bp 1.0 82.6
trnK-UUU-rps16_p4 CCTTTCAGGATCAGTCGTGG GAGAAAAAGGGGCATGTTGC 55.0 50.0 58.0 57.9 1718-1758 bp 1.0 82.6
trnK-UUU-rps16_p5 TCAGGATCAGTCGTGGTCTT CCCAATGAGCCGTTTATCGA 50.0 50.0 58.1 58.1 1228-1269 bp 1.0 82.5