Hotspot

trnH-GUG-psbA

LSC · 92-486 · 395 bp

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Pi 0.0575
MarkerSeek score 80.50
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
395
variable_sites
33
pi
0.0575
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.83
markerseek_score
80.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 GATCCACTTGGCTACATCCG TTCCCTCTAGACCTAGCTGC 55.0 55.0 58.1 57.9 390-465 bp 1.0 88.6
trnH-GUG-psbA_p2 CAATCCACTGCCTTGATCCA TTCCCTCTAGACCTAGCTGC 50.0 55.0 57.9 57.9 404-479 bp 1.0 88.4
trnH-GUG-psbA_p3 AATCCACTGCCTTGATCCAC TTCCCTCTAGACCTAGCTGC 50.0 55.0 57.9 57.9 403-478 bp 1.0 88.4
trnH-GUG-psbA_p4 ACAATCCACTGCCTTGATCC TTCCCTCTAGACCTAGCTGC 50.0 55.0 57.9 57.9 405-480 bp 1.0 88.4
trnH-GUG-psbA_p5 AAAAATGACATGGGCGAACG TTCCCTCTAGACCTAGCTGC 45.0 55.0 57.7 57.9 530 bp 0.25 46.6