Hotspot

rpl16

LSC · 81214-82690 · 1477 bp

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Pi 0.135
MarkerSeek score 85.00
Primer available yes
Label rpl16

Feature Metadata

label_name
rpl16
feature_type
gene
region
LSC
length_bp
1477
variable_sites
185
pi
0.135
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.93
markerseek_score
85.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl16_p1 ACACATCAATTCTCGGGCTC GGTGTTGAAGGAATTGCACG 50.0 50.0 58.0 57.9 2321 bp 1.0 90.0
rpl16_p2 ACACATCAATTCTCGGGCTC AAACCCGTATGGACACCCTA 50.0 50.0 58.0 58.0 2133 bp 0.5 59.4
rpl16_p3 ACACATCAATTCTCGGGCTC AACCCGTATGGACACCCTAA 50.0 50.0 58.0 58.0 2132 bp 0.5 59.4
rpl16_p4 TCTCAATTCTCGGGCAATCG AAACCCGTATGGACACCCTA 50.0 50.0 58.1 58.0 2391 bp 0.5 59.2
rpl16_p5 TCTCAATTCTCGGGCAATCG AACCCGTATGGACACCCTAA 50.0 50.0 58.1 58.0 2390 bp 0.5 59.2