Hotspot

ndhD

SSC · 116134-117636 · 1503 bp

Pi 0.481

MarkerSeek score 78.00

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1503
variable_sites: 542
pi: 0.481
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.75
markerseek_score: 78.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	GCCTGACGAACACAAACATG	AAACAACTCGAAGCATGGGT	50.0	45.0	58.0	58.0	1901 bp	0.5	59.6
ndhD_p2	GCCTGACGAACACAAACATG	GGCATGAAACAACTCGAAGC	50.0	50.0	58.0	58.0	1907 bp	0.5	59.5
ndhD_p3	GCCTGACGAACACAAACATG	TGAATCTGCTTGTCCGACAG	50.0	50.0	58.0	57.9	1954 bp	0.5	59.0
ndhD_p4	GCCTGACGAACACAAACATG	CTTGGCCCTATCGCATGAAT	50.0	50.0	58.0	58.1	2263 bp	0.5	58.9
ndhD_p5	ATTGAAGAAAGGGCCTGACG	AAACAACTCGAAGCATGGGT	50.0	45.0	58.2	58.0	1913 bp	0.5	58.5