Hotspot

ndhA

SSC · 120217-122408 · 2192 bp

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Pi 0.468
MarkerSeek score 79.20
Primer available yes
Label ndhA

Feature Metadata

label_name
ndhA
feature_type
gene
region
SSC
length_bp
2192
variable_sites
865
pi
0.468
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.84
markerseek_score
79.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhA_p1 TGCAGCTCGTACTGTTTGTT ATTGGGGGTTATCAGGACCA 45.0 50.0 58.1 58.0 2867 bp 0.5 59.5
ndhA_p2 TGCAGCTCGTACTGTTTGTT TGGGGGTTATCAGGACCAAT 45.0 50.0 58.1 58.0 2865 bp 0.5 59.5
ndhA_p3 TTGCAGCTCGTACTGTTTGT ATTGGGGGTTATCAGGACCA 45.0 50.0 58.1 58.0 2868 bp 0.5 59.5
ndhA_p4 TTGCAGCTCGTACTGTTTGT TGGGGGTTATCAGGACCAAT 45.0 50.0 58.1 58.0 2866 bp 0.5 59.5
ndhA_p5 TGCAGCTCGTACTGTTTGTT TAGGGGATCAGGGTGGTTTT 45.0 50.0 58.1 57.9 2489 bp 0.5 59.0