Hotspot

psaC-ndhE

SSC · 118014-118258 · 245 bp

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Pi 0.497
MarkerSeek score 71.40
Primer available yes
Label psaC-ndhE

Feature Metadata

label_name
psaC-ndhE
feature_type
igs
region
SSC
length_bp
245
variable_sites
90
pi
0.497
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.74
markerseek_score
71.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaC-ndhE_p1 ACCCATGCTTCGAGTTGTTT CACACACATCCCCCTTCAAT 45.0 50.0 58.0 57.8 962 bp 0.5 58.1
psaC-ndhE_p2 GCTTCGAGTTGTTTCATGCC CACACACATCCCCCTTCAAT 50.0 50.0 58.0 57.8 956 bp 0.5 58.0
psaC-ndhE_p3 CTGTCGGACAAGCAGATTCA CACACACATCCCCCTTCAAT 50.0 50.0 57.9 57.8 909 bp 0.5 57.6
psaC-ndhE_p4 CTGGCCAAGCTGCTAAAGTA CACACACATCCCCCTTCAAT 50.0 50.0 57.9 57.8 1424 bp 0.5 57.3
psaC-ndhE_p5 TGGCCAAGCTGCTAAAGTAG CACACACATCCCCCTTCAAT 50.0 50.0 57.9 57.8 1423 bp 0.5 57.3