Hotspot

rpl32-trnL-UAG

SSC · 113638-114728 · 1091 bp

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Pi 0.414
MarkerSeek score 80.00
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
1091
variable_sites
371
pi
0.414
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.82
markerseek_score
80.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GGCAGCGTTGAAAGCTTTTT GGAGAGCGGGGACTAATAGT 45.0 55.0 58.4 58.0 1758 bp 0.5 56.6
rpl32-trnL-UAG_p2 GGCAGCGTTGAAAGCTTTTT TGGAGAGCGGGGACTAATAG 45.0 55.0 58.4 58.0 1759 bp 0.5 56.5
rpl32-trnL-UAG_p3 GGCAGCGTTGAAAGCTTTTT AGCGTGTCTACCGATTTCAC 45.0 50.0 58.4 58.0 1205 bp 0.5 56.5
rpl32-trnL-UAG_p4 GGCAGCGTTGAAAGCTTTTT CAGCGTGTCTACCGATTTCA 45.0 50.0 58.4 58.0 1206 bp 0.5 56.5
rpl32-trnL-UAG_p5 GGCAGCGTTGAAAGCTTTTT CGGGGACTAATAGTGGGGAT 45.0 55.0 58.4 58.0 1752 bp 0.5 56.4