Hotspot

rpl2-rpl23

IRb · 85910-85927 · 18 bp

Back to Sicyos

Pi 0.0556
MarkerSeek score 81.90
Primer available yes
Label rpl2-rpl23

Feature Metadata

label_name
rpl2-rpl23
feature_type
igs
region
IRb
length_bp
18
variable_sites
1
pi
0.0556
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
81.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl2-rpl23_p1 CCACAACGATGCTGTCCATA CGCTTCAACCGGGTTATTCT 50.0 50.0 58.0 58.3 181 bp 1.0 78.1
rpl2-rpl23_p2 GACTGTCTACGGCTCCATTG CGCTTCAACCGGGTTATTCT 55.0 50.0 58.1 58.3 129 bp 1.0 77.9
rpl2-rpl23_p3 CCACAACGATGCTGTCCATA ATCATTACGCTTCAACCGGG 50.0 50.0 58.0 58.3 188 bp 1.0 77.6
rpl2-rpl23_p4 GACTGTCTACGGCTCCATTG ATCATTACGCTTCAACCGGG 55.0 50.0 58.1 58.3 136 bp 1.0 77.3
rpl2-rpl23_p5 TGCTCGGGGTAGAAGTTTTG CGCTTCAACCGGGTTATTCT 50.0 50.0 57.8 58.3 105 bp 1.0 77.2