Hotspot

petG-trnW-CCA

LSC · 66256-66371 · 116 bp

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Pi 0.0973
MarkerSeek score 81.60
Primer available yes
Label petG-trnW-CCA

Feature Metadata

label_name
petG-trnW-CCA
feature_type
igs
region
LSC
length_bp
116
variable_sites
11
pi
0.0973
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
81.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petG-trnW-CCA_p1 GGGGATCAGTTGGACCTTTG CAAAACCCGATGTCGTAGGT 55.0 50.0 58.2 57.9 173-176 bp 1.0 84.4
petG-trnW-CCA_p2 GGGGATCAGTTGGACCTTTG CCCGATGTCGTAGGTTCAAA 55.0 50.0 58.2 57.6 168-171 bp 1.0 82.3
petG-trnW-CCA_p3 GGGGATCAGTTGGACCTTTG TCCAAAACCCGATGTCGTAG 55.0 50.0 58.2 57.6 175-178 bp 1.0 82.1
petG-trnW-CCA_p4 GGGGATCAGTTGGACCTTTG ACGCTCTTAGTTCAGTTCGG 55.0 50.0 58.2 57.7 208-211 bp 1.0 81.3
petG-trnW-CCA_p5 GGGGATCAGTTGGACCTTTG CGCTCTTAGTTCAGTTCGGT 55.0 50.0 58.2 57.7 207-210 bp 1.0 81.3