Hotspot

petN-psbM

LSC · 29478-30102 · 625 bp

Back to Sicyos

Pi 0.0607
MarkerSeek score 79.30
Primer available yes
Label petN-psbM

Feature Metadata

label_name
petN-psbM
feature_type
igs
region
LSC
length_bp
625
variable_sites
37
pi
0.0607
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
79.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petN-psbM_p1 ATGGGGAAGAAGTGGACTCT ACGTAAAAACAATCAGTCAAAGTGA 50.0 32.0 57.6 57.9 665-676 bp 1.0 46.2
petN-psbM_p2 ATGGGGAAGAAGTGGACTCT ACGTAAAAACAATCAGTCAAAGTG 50.0 33.3 57.6 56.7 665-676 bp 1.0 46.2
petN-psbM_p3 ATGGGGAAGAAGTGGACTCT CGTAAAAACAATCAGTCAAAGTGA 50.0 33.3 57.6 56.4 664-675 bp 1.0 46.2
petN-psbM_p4 ATGGGGAAGAAGTGGACTCT ACGTAAAAACAATCAGTCAAAGT 50.0 30.4 57.6 55.3 665-676 bp 1.0 46.2
petN-psbM_p5 CTCGCTTGGGCTGCTTTAAT ACGTAAAAACAATCAGTCAAAGTGA 50.0 32.0 58.9 57.9 718-729 bp 1.0 45.7