Hotspot

trnK-rps16

LSC · 4173-4972 · 800 bp

Back to Sicyos

Pi 0.0566
MarkerSeek score 87.00
Primer available yes
Label trnK-rps16

Feature Metadata

label_name
trnK-rps16
feature_type
igs
region
LSC
length_bp
800
variable_sites
43
pi
0.0566
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.95
markerseek_score
87.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-rps16_p1 AGTCGCGGTCTTACAAACTC AGAAGGCAGGGGTTTTTACG 50.0 50.0 57.9 58.1 958-1024 bp 1.0 85.5
trnK-rps16_p2 GTCGCGGTCTTACAAACTCT AGAAGGCAGGGGTTTTTACG 50.0 50.0 57.9 58.1 957-1023 bp 1.0 85.5
trnK-rps16_p3 AGTCGCGGTCTTACAAACTC GGTGCTCAACCTACAGGAAC 50.0 55.0 57.9 58.2 995-1061 bp 1.0 84.4
trnK-rps16_p4 GTCGCGGTCTTACAAACTCT GGTGCTCAACCTACAGGAAC 50.0 55.0 57.9 58.2 994-1060 bp 1.0 84.4
trnK-rps16_p5 CAGTCGCGGTCTTACAAACT AGAAGGCAGGGGTTTTTACG 50.0 50.0 58.2 58.1 959-1025 bp 1.0 84.2