Hotspot

ndhA

SSC · 120056-122377 · 2322 bp

Back to Sibbaldianthe

Pi 0.00697

MarkerSeek score 63.80

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2322
variable_sites: 26
pi: 0.00697
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 63.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	TGACCATAATTCATGAACCCAGT	TGGGAGAAGTTGATCGTTGA	39.1	45.0	57.8	56.5	2411-2463 bp	1.0	45.1
ndhA_p2	TGACCATAATTCATGAACCCAGT	TGGGAGAAGTTGATCGTTGAA	39.1	42.9	57.8	57.2	2411-2463 bp	1.0	43.1
ndhA_p3	TGACCATAATTCATGAACCCAGT	ATGGGAGAAGTTGATCGTTGA	39.1	42.9	57.8	57.0	2412-2464 bp	1.0	42.7
ndhA_p4	TCATGAACCCAGTTACCATAGA	TGGGAGAAGTTGATCGTTGA	40.9	45.0	56.3	56.5	2401-2453 bp	1.0	42.7
ndhA_p5	TTCATGAACCCAGTTACCAT	TGGGAGAAGTTGATCGTTGA	40.0	45.0	54.2	56.5	2402-2454 bp	1.0	42.7