Hotspot

psaJ-rpl33

LSC · 67538-67983 · 446 bp

Back to Sibbaldianthe

Pi 0.0183

MarkerSeek score 59.30

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 446
variable_sites: 13
pi: 0.0183
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	TCCGGATGCGTTGATATTCC	GGACATCTTTACCCTTGGCC	50.0	55.0	57.8	58.2	492-499 bp	1.0	80.7
psaJ-rpl33_p2	TCCGGATGCGTTGATATTCC	TCTGGAAATGCCCGTTGATT	50.0	45.0	57.8	57.8	566-573 bp	1.0	80.3
psaJ-rpl33_p3	TGGCACCGGTAGTAAGTACT	GGACATCTTTACCCTTGGCC	50.0	55.0	57.8	58.2	562-569 bp	1.0	79.7
psaJ-rpl33_p4	TGGCACCGGTAGTAAGTACT	TCTGGAAATGCCCGTTGATT	50.0	45.0	57.8	57.8	636-643 bp	1.0	79.5
psaJ-rpl33_p5	GTGGCACCGGTAGTAAGTAC	TCTGGAAATGCCCGTTGATT	55.0	45.0	57.7	57.8	637-644 bp	1.0	79.0