Hotspot

psbE-petL

LSC · 64828-66091 · 1264 bp

Back to Sibbaldianthe

Pi 0.00833

MarkerSeek score 57.80

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1264
variable_sites: 17
pi: 0.00833
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 57.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	AGAACGTTCTCCTGTGCTTC	AAGTCGTATCTTGCTCAGGC	50.0	50.0	57.8	57.7	1339-1381 bp	1.0	80.4
psbE-petL_p2	GTTCTCCTGTGCTTCCAGAC	AAGTCGTATCTTGCTCAGGC	55.0	50.0	58.2	57.7	1334-1376 bp	1.0	80.1
psbE-petL_p3	TCTCCTGTGCTTCCAGACAT	AAGTCGTATCTTGCTCAGGC	50.0	50.0	58.4	57.7	1332-1374 bp	1.0	78.9
psbE-petL_p4	TTCTCCTGTGCTTCCAGACA	AAGTCGTATCTTGCTCAGGC	50.0	50.0	58.6	57.7	1333-1375 bp	1.0	77.1
psbE-petL_p5	AAGAACGTTCTCCTGTGCTT	AAGTCGTATCTTGCTCAGGC	45.0	50.0	57.4	57.7	1340-1382 bp	1.0	76.7