Hotspot

accD-psaI

LSC · 59003-59546 · 544 bp

Back to Sibbaldianthe

Pi 0.0208

MarkerSeek score 72.90

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 544
variable_sites: 19
pi: 0.0208
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	CGACTACACGCTTTCTTTCC	CCATTGCAATTGCCGGAAAT	50.0	45.0	56.9	58.0	651-680 bp	1.0	75.0
accD-psaI_p2	CGACTACACGCTTTCTTTCC	TTGCCGGAAATACTAAGCCC	50.0	50.0	56.9	58.0	642-671 bp	1.0	74.9
accD-psaI_p3	ACACGCTTTCTTTCCTTTGG	CCATTGCAATTGCCGGAAAT	45.0	45.0	56.8	58.0	646-675 bp	1.0	74.9
accD-psaI_p4	ACACGCTTTCTTTCCTTTGG	TTGCCGGAAATACTAAGCCC	45.0	50.0	56.8	58.0	637-666 bp	1.0	74.8
accD-psaI_p5	CGACTACACGCTTTCTTTCC	AGAAACCATTGCAATTGCCG	50.0	45.0	56.9	57.9	656-685 bp	1.0	74.6