Hotspot

trnC-GCA-petN

LSC · 27624-28458 · 835 bp

Back to Sibbaldianthe

Pi 0.0101

MarkerSeek score 63.10

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 835
variable_sites: 13
pi: 0.0101
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTTCCCCAGTTCAAATCCGG	AGAGTCCACTTCTTCCCCAT	50.0	50.0	58.1	57.6	920-952 bp	1.0	81.7
trnC-GCA-petN_p2	TTTCCCCAGTTCAAATCCGG	TACCATTAAAGCAGCCCAGG	50.0	50.0	58.1	57.6	871-903 bp	1.0	81.0
trnC-GCA-petN_p3	GCTATCATTTTGGCGGCATG	AGAGTCCACTTCTTCCCCAT	50.0	50.0	58.2	57.6	973-1005 bp	1.0	80.4
trnC-GCA-petN_p4	GCTATCATTTTGGCGGCATG	TACCATTAAAGCAGCCCAGG	50.0	50.0	58.2	57.6	924-956 bp	1.0	79.8
trnC-GCA-petN_p5	GGGGGACTGCAAATCCTTTT	AGAGTCCACTTCTTCCCCAT	50.0	50.0	58.4	57.6	938-970 bp	1.0	79.4