Hotspot

trnR-UCU-atpA

LSC · 10069-10726 · 658 bp

Back to Sibbaldianthe

Pi 0.0195

MarkerSeek score 61.30

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 658
variable_sites: 20
pi: 0.0195
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 61.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	AGCCTTCCAAGCTAACGATG	CGGCAGCTCAAATTAAAGCC	50.0	50.0	58.0	58.1	1411-1451 bp	1.0	83.0
trnR-UCU-atpA_p2	AGCCTTCCAAGCTAACGATG	GCGTATCCGGGAGATGTTTT	50.0	50.0	58.0	58.1	1661-1701 bp	1.0	82.8
trnR-UCU-atpA_p3	CCTAGCCTTCCAAGCTAACG	GCGTATCCGGGAGATGTTTT	55.0	50.0	58.1	58.1	1664-1704 bp	1.0	82.6
trnR-UCU-atpA_p4	AGCCTTCCAAGCTAACGATG	TGGAAAGAGCCGCGAAATTA	50.0	45.0	58.0	57.9	1621-1661 bp	1.0	82.5
trnR-UCU-atpA_p5	AGGGATCGTCTAGAAAGCGA	GCGTATCCGGGAGATGTTTT	50.0	50.0	57.9	58.1	1868-1908 bp	1.0	82.3