Hotspot

ycf1

SSC · 123310-129039 · 5730 bp

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Pi 0.00939

MarkerSeek score 48.80

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5730
variable_sites: 104
pi: 0.00939
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TTTTTGCATGTCGGAGTCCA	TGTTGGAACACCAGAACTTGT	45.0	42.9	58.0	57.9	1093-1099 bp	0.75	63.4
ycf1_p2	TTCAAGGTTTGCTTGGTCCA	TGTTGGAACACCAGAACTTGT	45.0	42.9	57.8	57.9	1030-1036 bp	0.75	62.5
ycf1_p3	GTTTTTGCATGTCGGAGTCC	TGTTGGAACACCAGAACTTGT	50.0	42.9	57.7	57.9	1094-1100 bp	0.75	61.0
ycf1_p4	GGTTTGCTTGGTCCACAGAT	TGTTGGAACACCAGAACTTGT	50.0	42.9	58.4	57.9	1025-1031 bp	0.75	60.7
ycf1_p5	TGCATGTCGGAGTCCAGATA	TGTTGGAACACCAGAACTTGT	50.0	42.9	58.2	57.9	1095 bp	0.25	39.6