Hotspot

rpl32-trnL-UAG

SSC · 112865-113472 · 608 bp

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Pi 0.0147

MarkerSeek score 69.30

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 608
variable_sites: 17
pi: 0.0147
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 69.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GTATTGGGCAGCGTTGAAAG	AGCGTGTCTACCGATTTCAC	50.0	50.0	57.7	58.0	725-813 bp	1.0	82.3
rpl32-trnL-UAG_p2	GTATTGGGCAGCGTTGAAAG	CAGCGTGTCTACCGATTTCA	50.0	50.0	57.7	58.0	726-814 bp	1.0	82.3
rpl32-trnL-UAG_p3	TCGCTTTCGACCGGTAATTC	AGCGTGTCTACCGATTTCAC	50.0	50.0	58.4	58.0	688-776 bp	1.0	81.7
rpl32-trnL-UAG_p4	TCGCTTTCGACCGGTAATTC	CAGCGTGTCTACCGATTTCA	50.0	50.0	58.4	58.0	689-777 bp	1.0	81.7
rpl32-trnL-UAG_p5	GTATTGGGCAGCGTTGAAAG	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	57.7	57.9	735-823 bp	1.0	81.4