Hotspot

ndhF-rpl32

SSC · 112072-112705 · 634 bp

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Pi 0.00731

MarkerSeek score 63.80

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 634
variable_sites: 9
pi: 0.00731
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 63.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GAAGCCCCGCTCCTATTAAC	CTTTCAACGCTGCCCAATAC	55.0	50.0	58.1	57.7	786-804 bp	1.0	79.8
ndhF-rpl32_p2	GTTGGAAAAAGCAGAAGCCC	CTTTCAACGCTGCCCAATAC	50.0	50.0	57.8	57.7	799-817 bp	1.0	79.4
ndhF-rpl32_p3	CTGGAAGTGGAATGAACGGT	CTTTCAACGCTGCCCAATAC	50.0	50.0	57.8	57.7	759-777 bp	1.0	79.3
ndhF-rpl32_p4	AGAAGCCCCGCTCCTATTAA	CTTTCAACGCTGCCCAATAC	50.0	50.0	58.2	57.7	787-805 bp	1.0	79.1
ndhF-rpl32_p5	CGTTGGAAAAAGCAGAAGCC	CTTTCAACGCTGCCCAATAC	50.0	50.0	58.2	57.7	800-818 bp	1.0	78.8