Hotspot

petA-psbJ

LSC · 62794-63478 · 685 bp

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Pi 0.0105

MarkerSeek score 66.00

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 685
variable_sites: 14
pi: 0.0105
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 66.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ATCCATTACGTGTCCAAGGC	GCCGATACTACTGGAAGGAT	50.0	50.0	58.0	56.2	895-984 bp	1.0	70.4
petA-psbJ_p2	CATTACGTGTCCAAGGCCTT	GCCGATACTACTGGAAGGAT	50.0	50.0	58.2	56.2	892-981 bp	1.0	69.3
petA-psbJ_p3	TGAGAAGGTTCAGTTGGCTG	GCCGATACTACTGGAAGGAT	50.0	50.0	57.7	56.2	810-899 bp	1.0	68.9
petA-psbJ_p4	ATCCATTACGTGTCCAAGGC	GCCGATACTACTGGAAGGATT	50.0	47.6	58.0	57.0	895-984 bp	1.0	68.5
petA-psbJ_p5	ATCCATTACGTGTCCAAGGC	GCCGATACTACTGGAAGGATTC	50.0	50.0	58.0	58.1	895-984 bp	1.0	68.1