Hotspot

ndhC-trnV-UAC

LSC · 50860-51452 · 593 bp

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Pi 0.0187
MarkerSeek score 65.40
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
593
variable_sites
18
pi
0.0187
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.92
markerseek_score
65.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 CATATTCGTAAAGCAGCAGCA GCACGTTGGGTCTTTGAAAC 42.9 50.0 57.0 58.2 678-759 bp 1.0 69.1
ndhC-trnV-UAC_p2 CATATTCGTAAAGCAGCAGCA AGAAGGTCTACGGTTCGAGT 42.9 50.0 57.0 57.8 614-695 bp 1.0 69.0
ndhC-trnV-UAC_p3 CATATTCGTAAAGCAGCAGCA TGGGTCTTTGAAACGGTTCA 42.9 45.0 57.0 57.6 672-753 bp 1.0 67.1
ndhC-trnV-UAC_p4 TCATATTCGTAAAGCAGCAGC GCACGTTGGGTCTTTGAAAC 42.9 50.0 56.8 58.2 679-760 bp 1.0 66.9
ndhC-trnV-UAC_p5 TCATATTCGTAAAGCAGCAGC AGAAGGTCTACGGTTCGAGT 42.9 50.0 56.8 57.8 615-696 bp 1.0 66.9