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psaA-ycf3

LSC · 41455-42221 · 767 bp

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Pi 0.00823

MarkerSeek score 66.50

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 767
variable_sites: 12
pi: 0.00823
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 66.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	TTACGGGATCCCTATCGACC	ATAGCGCTTACTCCGGGTAA	55.0	50.0	58.1	58.3	876-894 bp	1.0	79.6
psaA-ycf3_p2	TTACGGGATCCCTATCGACC	TAGCGCTTACTCCGGGTAAT	55.0	50.0	58.1	58.3	875-893 bp	1.0	79.6
psaA-ycf3_p3	GCCCATTCCTCGAAAGAAGT	ATAGCGCTTACTCCGGGTAA	50.0	50.0	57.9	58.3	898-916 bp	1.0	79.3
psaA-ycf3_p4	GCCCATTCCTCGAAAGAAGT	TAGCGCTTACTCCGGGTAAT	50.0	50.0	57.9	58.3	897-915 bp	1.0	79.3
psaA-ycf3_p5	TTACGGGATCCCTATCGACC	GCGCTTACTCCGGGTAATTA	55.0	50.0	58.1	57.5	873-891 bp	1.0	77.9