Hotspot

trnT-GGU-psbD

LSC · 31106-32414 · 1309 bp

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Pi 0.0191

MarkerSeek score 65.80

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1309
variable_sites: 38
pi: 0.0191
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 65.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GCCCTTTTAACTCAGCGGTA	GGCCAGACCAACCTACAAAA	50.0	50.0	57.9	58.0	1454-1487 bp	1.0	83.4
trnT-GGU-psbD_p2	GCCCTTTTAACTCAGCGGTA	TAGGCCAGACCAACCTACAA	50.0	50.0	57.9	58.0	1456-1489 bp	1.0	83.2
trnT-GGU-psbD_p3	GGCGTAAGTCATCGGTTCAA	GGCCAGACCAACCTACAAAA	50.0	50.0	58.3	58.0	1417-1450 bp	1.0	82.0
trnT-GGU-psbD_p4	GGCGTAAGTCATCGGTTCAA	TAGGCCAGACCAACCTACAA	50.0	50.0	58.3	58.0	1419-1452 bp	1.0	81.9
trnT-GGU-psbD_p5	GCCCTTTTAACTCAGCGGTA	ATAGGCCAGACCAACCTACA	50.0	50.0	57.9	57.7	1457-1490 bp	1.0	81.3