Hotspot

psbM-trnD-GUC

LSC · 29337-29934 · 598 bp

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Pi 0.00872

MarkerSeek score 65.30

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 598
variable_sites: 9
pi: 0.00872
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 65.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	CAATCACCTTGGCTGACTGT	TTCAATTGGTCAGAGCACCG	50.0	50.0	58.1	58.5	719-766 bp	1.0	80.4
psbM-trnD-GUC_p2	TCAATCACCTTGGCTGACTG	TTCAATTGGTCAGAGCACCG	50.0	50.0	57.8	58.5	720-767 bp	1.0	79.6
psbM-trnD-GUC_p3	TCACCTTGGCTGACTGTTTT	TTCAATTGGTCAGAGCACCG	45.0	50.0	57.6	58.5	716-763 bp	1.0	77.7
psbM-trnD-GUC_p4	CAATCACCTTGGCTGACTGT	GTTCAATTGGTCAGAGCACC	50.0	50.0	58.1	57.0	720-767 bp	1.0	76.2
psbM-trnD-GUC_p5	ATCACCTTGGCTGACTGTTT	TTCAATTGGTCAGAGCACCG	45.0	50.0	57.3	58.5	717-764 bp	1.0	75.8