Hotspot

petN-psbM

LSC · 28113-29231 · 1119 bp

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Pi 0.00824

MarkerSeek score 66.70

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1119
variable_sites: 18
pi: 0.00824
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 66.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	AGTAAATCTCGCTTGGGCTG	CAGTCAGCCAAGGTGATTGA	50.0	50.0	58.0	57.8	1221-1311 bp	1.0	80.8
petN-psbM_p2	AGTAAATCTCGCTTGGGCTG	AAAACAGTCAGCCAAGGTGA	50.0	45.0	58.0	57.6	1225-1315 bp	1.0	78.8
petN-psbM_p3	ATGGGGAAGAAGTGGACTCT	CAGTCAGCCAAGGTGATTGA	50.0	50.0	57.6	57.8	1161-1251 bp	1.0	78.4
petN-psbM_p4	AGTAAATCTCGCTTGGGCTG	AAACAGTCAGCCAAGGTGAT	50.0	45.0	58.0	57.3	1224-1314 bp	1.0	77.0
petN-psbM_p5	AGTAAATCTCGCTTGGGCTG	AACAGTCAGCCAAGGTGATT	50.0	45.0	58.0	57.3	1223-1313 bp	1.0	77.0