Hotspot

trnR-UCU-atpA

LSC · 9660-10344 · 685 bp

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Pi 0.0156

MarkerSeek score 65.70

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 685
variable_sites: 19
pi: 0.0156
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 65.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	GGTTCAAATCCTATTGGACGC	AGACATTTACCGAGGAAGCG	47.6	50.0	57.3	57.7	772-854 bp	1.0	70.0
trnR-UCU-atpA_p2	GGTTCAAATCCTATTGGACGC	GAGGAAGCGGAAACCCTTTT	47.6	50.0	57.3	58.4	761-843 bp	1.0	69.3
trnR-UCU-atpA_p3	GGTTCAAATCCTATTGGACGC	TCAGGACCAAATGGAACGTT	47.6	45.0	57.3	57.4	729-811 bp	1.0	67.3
trnR-UCU-atpA_p4	GGTTCAAATCCTATTGGACGC	TTCAGGACCAAATGGAACGT	47.6	45.0	57.3	57.4	730-812 bp	1.0	67.3
trnR-UCU-atpA_p5	TGGATAGGACAGAGGTCTTCT	AGACATTTACCGAGGAAGCG	47.6	50.0	56.9	57.7	807-889 bp	1.0	67.0