Hotspot

rpl16

LSC · 76783-78134 · 1352 bp

Back to Shivparvatia

Pi 0.0541
MarkerSeek score 77.10
Primer available yes
Label rpl16

Feature Metadata

label_name
rpl16
feature_type
gene
region
LSC
length_bp
1352
variable_sites
71
pi
0.0541
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
77.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl16_p1 AGCATATCGGCGATTGCTAG CAAGAACATCCTCGGGTGTC 50.0 55.0 58.0 58.3 2165-2211 bp 1.0 83.9
rpl16_p2 CATATCGGCGATTGCTAGCT CAAGAACATCCTCGGGTGTC 50.0 55.0 58.0 58.3 2163-2209 bp 1.0 83.9
rpl16_p3 GAGCATATCGGCGATTGCTA CAAGAACATCCTCGGGTGTC 50.0 55.0 58.0 58.3 2166-2212 bp 1.0 83.9
rpl16_p4 TTTTCTCAATTCCCGGGCAA CAAGAACATCCTCGGGTGTC 45.0 55.0 58.0 58.3 2390-2436 bp 1.0 83.6
rpl16_p5 TTTTTCTCAATTCCCGGGCA CAAGAACATCCTCGGGTGTC 45.0 55.0 58.0 58.3 2391-2437 bp 1.0 83.6