Hotspot

ndhF

SSC · 104947-107178 · 2232 bp

Back to Shivparvatia

Pi 0.361

MarkerSeek score 69.20

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2232
variable_sites: 221
pi: 0.361
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.27
markerseek_score: 69.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	GGAATCGTCCAACGCGATAT	ACTAAAAGATGCAACCCGAA	50.0	40.0	58.2	55.3	2958 bp	0.5	36.4
ndhF_p2	GGAATCGTCCAACGCGATAT	ACTAAAAGATGCAACCCGAAA	50.0	38.1	58.2	56.1	2958 bp	0.5	34.8
ndhF_p3	GGAATCGTCCAACGCGATAT	ACTAAAAGATGCAACCCGAAAT	50.0	36.4	58.2	56.6	2958 bp	0.5	31.0
ndhF_p4	AAAAAGGCTTTTCACCCTCT	ACATACTAAACCACGCCGAT	40.0	45.0	55.3	57.0	2942 bp	0.5	30.8
ndhF_p5	AAAAAGGCTTTTCACCCTCT	TGTACATACTAAACCACGCCG	40.0	47.6	55.3	58.1	2945 bp	0.5	29.7