Hotspot

clpP

LSC · 65747-67769 · 2023 bp

Back to Shivparvatia

Pi 0.0523
MarkerSeek score 73.70
Primer available yes
Label clpP

Feature Metadata

label_name
clpP
feature_type
gene
region
LSC
length_bp
2023
variable_sites
104
pi
0.0523
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
73.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
clpP_p1 CGTTAACGCTACCAATGGGA GCCGGGATCATTCAATACGA 50.0 50.0 58.0 57.8 2920-2928 bp 1.0 84.0
clpP_p2 CTAGTACATGTTCCTCGGCG ACGAACATACCCTGTCTCCA 55.0 50.0 57.9 58.1 2857-2902 bp 1.0 83.6
clpP_p3 CTAGTACATGTTCCTCGGCG TCGGATCGGTTATAGTCCCC 55.0 55.0 57.9 58.1 2937-2982 bp 1.0 83.4
clpP_p4 TGACCCTGTTAGTCCGTTCT CGATACCAAGGCAAACCCAT 50.0 50.0 58.0 58.2 2970-2978 bp 1.0 83.4
clpP_p5 CGTTAACGCTACCAATGGGA CGATACCAAGGCAAACCCAT 50.0 50.0 58.0 58.2 2889-2897 bp 1.0 83.4