Hotspot

trnL-UAG-ccsA

SSC · 108878-109016 · 139 bp

Back to Shivparvatia

Pi 0.0853

MarkerSeek score 77.40

Primer available yes

Label trnL-UAG-ccsA

Feature Metadata

label_name: trnL-UAG-ccsA
feature_type: igs
region: SSC
length_bp: 139
variable_sites: 11
pi: 0.0853
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 77.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAG-ccsA_p1	TAGACACGCTGCTCTTAGGA	TGAGTCAAAATATGTTCTAAAGTCGA	50.0	30.8	57.9	56.9	225-235 bp	1.0	47.3
trnL-UAG-ccsA_p2	GTAGACACGCTGCTCTTAGG	TGAGTCAAAATATGTTCTAAAGTCGA	55.0	30.8	57.8	56.9	226-236 bp	1.0	47.3
trnL-UAG-ccsA_p3	GGTAGACACGCTGCTCTTAG	TGAGTCAAAATATGTTCTAAAGTCGA	55.0	30.8	57.8	56.9	227-237 bp	1.0	47.2
trnL-UAG-ccsA_p4	AAATTGGTAGACACGCTGCT	TGAGTCAAAATATGTTCTAAAGTCGA	45.0	30.8	57.8	56.9	232-242 bp	1.0	47.1
trnL-UAG-ccsA_p5	AATTGGTAGACACGCTGCTC	TGAGTCAAAATATGTTCTAAAGTCGA	50.0	30.8	58.3	56.9	231-241 bp	1.0	47.1